The miniprotean tetra cell runs up to four mini gels in as little as 15 minutes. It requires less than 200ml of transfer buffer for western, southern, and northern transfers. How to perform a 7 minute protein transfer with the iblot. The benchpro 4100 can minimize the time and manual labor that comes about with western blotting. Xcell surelock minicell with xcell ii blot module kit ei0002. The xcell ii blot module is a minigel wettransfer module for the xcell surelock or. It requires less than 200 ml of transfer buffer for western, southern, and northern transfers. Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user. If the lid is removed, the electrical connection will be broken. The novex semidry blotter is designed to be operated with the lid in place. Instructions are provided below for blotting nupage gels using the xcell ii blot module. Jan 25, 2020 eyeos user manual free download as pdf file. Upgrade the xcell ii minicell conversion of an xcell ii into an xcell surelock is easy with the purchase of a surelock retrofit kit ei0020 consisting of a surelock mini.
With the introduction of the new xcell surelock minicell, the tradition continues. Nupage electrophoresis system from invitrogen biocompare. Aug 21, 2019 the xcell ii blot module comes from invitrogen, which now markets the novex range of protein electrophoresis products. Western blotting using the invitrogen nupage novex bis. The xcell line of minicells has always distinguished itself by being simple, sturdy, and convenient. The xcell surelock minicell can be easily adapted for blotting by inserting the current xcell ii blot module in place of the gelbuffer core assembly. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Invitrogen powerease 500 electrophoresis with xcell sure. Anode core, cathode core, sponge pads 8pack, and instruction manual. Choosing a gel for your application to obtain the best results for your application, it is important to choose the. This electrophoresis power supply is microprocessor controlled with flexible programming, and features a large lcd display.
Launch your terminal and run following command to install lamp. The xcell surelock minicell and xcell ii blot module includes the xcell surelock minicell for running novex minigels and the xcell ii blot module for blotting novex minigels. Yanmar c80r service manual buderus ecomatic hs manuals buderus ecomatic service manual rvice manual lombardini engine english. If this item is no longer available be sure to shop our full selection of electrophoresis power supply systems at. Invitrogen novex power ease 500 electrophoresis power supply buy now.
Invitrogen novex xcell ii blot vertical module 1 unit electrophoresis, western blotting and elisa. Transfer cell, or in references 23 if your lab is equipped with the invitrogens xcell ii blot module. Its unique design allows the user to quickly and easily perform simultaneous vertical electrophoresis of 1 to 4 midigels without leaking. Ordering information for the most widely used products is. Novex bistris gels have a shelf life of 12 months when stored at 425c. Here is an archived copy of the invitrogen 3insert multisite gateway manual, version d, 30707.
Invitrogen sponge pad for blotting for the xcell surelock mini blot module 260. Invitrogen novex power ease 500 electrophoresis power supply for sale online. Western blotting using nitrocellulose membranes life technologies. This blot module can be used for the transfer step in western, southern or northern blotting.
Used invitrogen benchpro 4100 card processing station 415. Purelink pcr purification kit thermo fisher scientific. Nupage technical guide thermo fisher scientific mafiadoc. Invitrogen offers three types of blotting membrane and filter. Powerease 500 ei8600, powerease 500 ei8700, powerease 500 ei8675. Nupage antioxidant the nupage antioxidant is added to the transfer buffer when blotting reduced proteins to prevent reoxidation and maintain proteins in a reduced state see page. Invitrogen mini gel tank and blot module set with two blot. Get the best deals on gel electrophoresis systems when you shop the largest online selection at.
The figure below shows a dna ladder lane 1, the pcr product before purification lane 2, and the pcr product after purification lane 3. Invitrogen xcell surelock novex mini cell w xcell ii blot module guaranteed. Eyeos manual pdf eyeos user manual free download as pdf file. The incorporation of a gel tension wedge in place of the rear wedge used on earlier models makes the xcell surelock the easiesttouse minicell available. Cast gels, visit our web site at or contact technical service see page 66. We have 1 invitrogen qubit 4 manual available for free pdf download. Find stepbystep protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods. Ic720a manual pdf this is the service manual for ica. Buderus ecomatic user ee download 1 my ms news live tile is not showing the latest news, so 300 who can help with solution kevin suing when i try. How to set up a western part 2 running the gel on the. May 14, 2015 invitrogen xcell sure lock minicell electrophoresis system.
Precast gels are capable of resolving proteins in the range of 2500 kda and nucleic acids in the range of 103000 bp. The xcell ii blot module from invitrogen biocompare. The novex semidry blotter is safe to use when operated in accordance with the instructions in this manual. Invitrogen manual version d link updated august 2017. Invitrogen xcell ii blot module electrophoresis block e19051. View and download invitrogen powerease 500 instructions for use manual online. Not intended for any animal or human therapeutic or diagnostic use. Xcell ii blot module the xcell ii blot module is a simple apparatus designed for blotting of minigels and is easily inserted into the xcell surelock minicell in place of the gelbuffer core assembly. Invitrogen xcell sure lock minicell electrophoresis. Gel tension wedge 1 each buffer dam 1 each surelock minicell lid 1 each xcell surelock minicell with xcell ii blot.
User manual corporate headquarters invitrogen corporation 1600 faraday avenue carlsbad, ca 92008 t. The invitrogen xcell4 surelock midicell electrophoresis system is built with a tough plastic material to boost durability and features a specially designed lid to enhance user safety. Invitrogen powerease 500 instructions for use manual pdf. The xcell ii blot module is a semiwet system for blotting up to two mini. How to start your western with the xcell surelock system for. Life technologies novex xcell surelock ei0001 max volt 1500vdc 75w new. We originally had a live link to the invitrogen site, but after this link broke it seemed easier to hardarchive a copy. This system can become an essential unit in the lab as western analysis of proteins is essential due to its high sensitivity and specificity. Optional equipment includes the xcell ii blot module for the transfer and the powerease 500 power programmable power supply.
Xcell surelock minicell electrophoresis system thermo. This website uses cookies to improve your experience. Xcell surelock minicell with xcell ii blot module kit. Designed with flexibility in mind, the miniprotean tetra cell can. For more information on the xcell ii blot module, refer to. For more information on the xcell ii blot module, refer to the manual im9051 available at or contact technical support see page 56. Do not attempt to use the blotter without the lid in place. Invitrogen powerease 500 electrophoresis with xcell sure lock. Install guide eyeos 2 cloud computing os on windows v global. Invitrogen novex xcell ii blot vertical module 1 unit. Invitrogen xcell ii blot module 1 unit western blotting. The pcr product was analyzed by agarose gel electrophoresis and shows efficient purification of a 965bp pcr product from a 232bp pcr fragment and primers. The xcell surelock minicell and xcell ii blot module set is an electrophoresis and blotting system for mini gels. The tetra cell is easy to assemble, leakproof, and reliable, providing reproducible, superior performance.
See all available electrophoresis chamber systems how the system works the xcell surelock minicell holds gels firmly with a. For detailed instructions, refer to the nupage technical guide available. Fits neatly into the xcell surelock and xcell ii minicells in place of the gelbuffer core assembly. Mini transblot module number of blots 12 blotting area 10 x 7.
Invitrogen xcell surelock minicell and xcell ii blot. Invitrogen mini gel tank and blot module set provides all of the necessary devices for protein separation and western blotting using novex gels, nupage gels, and bolt bistris plus gels brand. Get the best deals on gel electrophoresis systems when you shop the largest online. How to perform a traditional wet protein transfer using the xcell surelock blot module duration. The module has rails to guide the unit into the minicell. The xcell ii blot module can be used to perform western. Invitrogen qubit 4 manuals manuals and user guides for invitrogen qubit 4. Make offer biorad miniprotean gel casting stand with casting frames. Invitrogen xcell ii blot module 1 unit western blotting reagents and kits. Instead we use our old biorad transfer apparatus with the nupage transfer buffer. Pdf western blotting using the invitrogen nupage novex. This power supply comes with 8 preprogrammed methods and can store up to 4 user defined protocols. Invitrogen xcell ii blot module 1 unit western blotting reagents.
The xcell ii blot module allows you to easily transfer proteins or nucleic acids for western, southern, and northern transfers from mini gels to membranes with less than 200 ml of transfer buffer. Invitrogen xcell sure lock minicell electrophoresis system. Protein transfer technical handbook thermo fisher scientific. The xcell ii blot module includes anode core, cathode core, sponge pads 8pack, and instruction manual. Pdf western blotting using the invitrogen nupage novex bis. Instructions are provided below for blotting novex precast gels using the xcell ii blot module. The xcell ii blot module allows you to easily transfer proteins or nucleic acids from minigels to membranes. How to perform a traditional wet protein transfer using the xcell. It fits neatly into the xcell surelock and xcell ii minicells in place of the gelbuffer core assembly. Its unique design allows the user to quickly and easily perform simultaneous vertical electrophoresis of 1 to 4.
Biorads v3 western workflow facilitates speed and validation at each step of a western blotting experiment from running gels to quantifying proteins. Zoom dual power supply a twoinone programmable power supply for high voltagelow current and low voltagehigh current electrophoresis applications catalog numbers zp1, zp2 revision date 23 march 2012 publication part number 250863. Install guide eyeos 2 cloud computing os on windows v global open versity cloud computing handson labs training manual. Well assume youre ok with this, but you can optout if you wish. Cell vertical 10x10cm gel cassette surelock mini cell system with manual invitrogen xcell surelock minicell. How to start your western with the xcell surelock system for nupage gels. Ordering information for the most widely used products is provided below. The incorporation of a gel tension wedge, in place of the rear wedge used on. The xcell ii blot module comes from invitrogen, which now markets the novex range of protein electrophoresis products. Novex xcell ii electrophoresis cell with ei9051 blot. The invitrogen benchpro 4100 is a western blot processing system for the analysis of proteins. In this video life technologies staff scientist, david bourdon ph.
Apr 04, 2012 how to perform a traditional wet protein transfer using the xcell surelock blot module duration. Running the gel on the xcell surelock system thermo fisher scientific. How to set up a western part 2 running the gel on the xcell. Invitrogen mini gel tank and blot module set with two. Bolt bistris plus gels, nupage gels, novex mini gels.
Invitrogen xcell4 surelock midicell electrophoresis system. The invitrogen powerease 500 power supply with xcell surelock minicell is simple to use, sturdily constructed, leak free and convenient. Invitrogen xcell surelock minicell and xcell ii blot module. The xcell ii blot module is a minigel wettransfer module for the xcell surelock or xcell ii systems. Invitrogen complete kit xcell4 surelock midicell nice cond, novex life biorad. It includes the xcell surelock minicell for running invitrogen mini gels and the xcell ii blot module for blotting up to two mini gels at a time. Novex xcell ii electrophoresis cell with ei9051 blot module. Instead of being a complete and separate piece of apparatus for blotting, the xcell ii blot module is designed to fit into either the xcell surelock minicell or the xcell ii minicell, in place of the gelbuffer core assembly an older version of the. A detailed stepbystep protocol of the transfer procedure can be found in reference 4 if you use the biorad mini transblot electrophoretic transfer cell, or in references 23 if your lab is equipped with the invitrogens xcell ii blot module.
399 495 1564 1527 624 676 1208 1442 351 1064 293 1388 793 277 1470 1201 1364 759 358 1376 1411 454 604 537 936 1214 1389 75 124 1463